Holy Trinity competes in the Catholic Athletic Association.

Baseball/Softball
Holy Trinity offers baseball/softball for boys and girls in sixth grade. The season runs from mid April through mid May.

Basketball
Holy Trinity offers basketball for boys and girls in fifth and sixth grade.

Soccer
Holy Trinity offers soccer for both boys and girls in grades five and six. The season runs from the first week of September through mid October. 

Swimming
The Aqua Saints is a swim team made up of boys and girls from grades Kindergarten through six. It is a combined team of students from Holy Trinity, St. John Vianney and St. Michael schools. Practices are held in the Central Square pool in South St. Paul. Meets are scheduled throughout February and March and are held in area pools. The finals will be held at The Aquatic Center at the University of Minnesota. The swimming program is run through the Catholic Athletic Association.

Volleyball
Holy Trinity offers volleyball in fifth and sixth grade. The season runs from mid September through mid October.

Running Club
Holy Trinity Running Club is open to girls and boys in grades 2 though 6. Beginning in late March, the club meets after school for training sessions in preparation for the Get in Gear 2K Fit For Fun Run held the last Saturday of April at Minnehaha Park in Minneapolis.

Intramural Activities
Intramural volleyball is open to boys and girls in grades 3 and 4. Sessions are held in the evening during November. Registration begins in October.

Intramural basketball is open to girls and boys in grades 3 and 4. Sessions are held on in the evening during January and February. Registration begins in December.

Intramural Floor Hockey is open to girls and boys in grades 3 and 4. Sessions are held in the evenings during March. Registration begins in February.